The guideline provides a practical manual for cultivating Gynostemma pentaphyllum (Giảo cổ lam) under organic standards in Vietnam’s mountainous regions. It highlights the plant’s medicinal and economic value, emphasizing its suitability for cool, humid environments and semi-shaded conditions. The document outlines key steps from selecting high-quality cuttings and preparing organic-compliant soil to propagation techniques such as stem cutting and nursery management. Field cultivation includes land preparation, planting density, trellis construction, irrigation, and organic fertilization. Pest and disease management follows ecological principles, using biological agents, botanical extracts, and biodiversity conservation instead of synthetic chemicals. Harvesting is conducted 4–5 months after planting, with multiple harvest cycles per year. Post-harvest processes include cleaning, cutting, drying, and proper storage to maintain quality. The guideline also stresses waste recycling into organic fertilizers, promoting circular agriculture, sustainable livelihoods, and value chain development for medicinal plants.