The Handbook for the Evaluation of Agroecology (March 2019 edition) was developed to help development practitioners and researchers systematically assess the agro-environmental, economic, and social impacts of agroecological practices while identifying the enabling factors and constraints affecting their adoption and scaling. The handbook’s methodology is based on comparative analysis and a multi-scale systems approach, structured around two core components: diagnostic analysis of farming systems and the design of appropriate monitoring and evaluation (M&E) frameworks. Its comprehensive guidance sheets provide detailed criteria and indicators spanning from plot level to value chains, covering soil health, resource-use efficiency, food security, climate resilience, and women’s empowerment. As such, the handbook serves as a foundation for generating robust scientific evidence to support donors, inform nature-positive public policies, and strengthen the long-term self-reliance of farming households.